Efficient downregulation ofalb1gene using an AMA1-based episomal expression of RNAi construct inAspergillus fumigatus
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چکیده
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nano-rods zno as an efficient catalyst for the synthesis of chromene phosphonates, direct amidation and formylation of amines
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RNAi-mediated knockdown of mouse melanocortin-4 receptor in vitro and in vivo, using an siRNA expression construct based on the mir-187 precursor
RNA interference (RNAi) is a powerful tool for the study of gene function in mammalian systems, including transgenic mice. Here, we report a gene knockdown system based on the human mir-187 precursor. We introduced small interfering RNA (siRNA) sequences against the mouse melanocortin-4 receptor (mMc4r) to alter the targeting of miR-187. The siRNA-expressing cassette was placed under the contro...
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An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi
RNA interference (RNAi) mediates gene silencing in many eukaryotes and has been widely used to investigate gene functions. A common method to induce sustained RNAi is introducing plasmids that synthesize short hairpin RNAs (shRNAs) using Pol III promoters. While these promoters synthesize shRNAs and elicit RNAi efficiently, they lack cell specificity. Monitoring shRNA expression levels in indiv...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2007
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2007.00680.x